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Primary Antibodies

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Catalog #1: bsm-33293M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bsm-33293M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

HeLa cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-33042M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33042M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33042M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33042M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33042M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33042M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33042M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33187M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

A431 cell lysate probed with ACTA2 (3F9) Monoclonal Antibody (bsm-33187M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:400 overnight at 4\u00b0C, followed by incubation with a secondary antibody, DAB and counter-staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Small intestine lysates; Lane 6: Mouse NIH\/3T3 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Rat Uterus lysates; Lane 9: Human A549 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human A431 cell lysates; Lane 12: Human HepG2 cell lysates probed with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33187M

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33187M-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33187M-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187M-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33187M-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33035M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

NIH\/3T3 cells probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
HeLa cell lysate probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
A431 cell lysate probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA (3G1) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:400 overnight at 4\u00b0C, followed by operating according to DAB staining.

Catalog #1: bsm-33035M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33035M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33035M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33035M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33170M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33170M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Lane 1: Raji; Lane 2: U87MG cell lysate probed with Vimentin (3B7) Monoclonal Antibody, unconjugated (bsm-33170M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: HeLa (30ug); Lane 2: HeLa KO Vimentin lysate (30ug) probed with Vimentin (3B7) Monoclonal Antibody, unconjugated (bsm-33170M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Vimentin(3B7) ) Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Vimentin(3B7) ) Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33170M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33004M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33216M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33216M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33117M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33117M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

HepG2 cell lysate probed with MEK2 (4C3) Monoclonal Antibody (bsm-33216M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 (4C3) Monoclonal Antibody, Unconjugated (bsm-33216M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33216M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: A431; Lane 3: K562 cell lysate probed with NFKB p65 (7G6) Monoclonal Antibody, unconjugated (bsm-33117M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: Rela-KO cell lysates; Lane 2: Hela cell lysates probed with NFKB p65 (7G6) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: RELE-KO cell lysates; Lane 2: Hela cell lysates probed with NFKB p65 Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human MOLT-4 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Mouse NIH\/3T3 cell lysates probed with NFKB p65 Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33117M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate; Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate probed with His tag (6E6) Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with His tag Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:200000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33004M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33033M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bsm-33004M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Lane 1: A549 Cell lysates; Lane 2: 293T Cell lysates; probed with GAPDH (4F8) Monoclonal Antibody, unconjugated (bsm-33033M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Lovor cell lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hyperpituitarism lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Testis lysates; Lane 6: Rat Testis lysates; Lane 7: Mouse Kidney lysates; Lane 8: Human HUVEC cell lysates; Lane 9: Human A549 cell lysates; Lane 10: Human MCF-7 cell lysates probed with GAPDH Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33033M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33033M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bsm-33040M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33040M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33041M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33041M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

MOLT-4 cell lysates probed with Lamin B (9C11) Monoclonal Antibody, unconjugated (bsm-33040M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Rat Pancreas tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Thymus tissue lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Molt-4 cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human U-2os cell lysates; Lane 8: Human THP-1 cell lysates probed with Lamin B Monoclonal Antibody, Unconjugated (bsm-33040M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33040M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1\uff1aHuman 293T cell lysates; Lane 2: Mouse Cerebellum lysates; Lane 3:Human Jurkat cell lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HeLa cells were stained with beta 1 Tubulin (5F7) Monoclonal Antibody, Unconjugated(bsm-33041M) at 1:50 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Mouse IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Catalog #1: bsm-33041M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

DU145 cell lysates probed with p53 (8D6) Monoclonal Antibody, unconjugated (bsm-33058M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (P53(8D6)) Monoclonal Antibody, Unconjugated (bsm-33058M) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded (human esophageal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (P53(8D6)) Monoclonal Antibody, Unconjugated (bsm-33058M) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-33058M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33101M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33058M-Biotin

Applications: WB

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33058M-HRP

Applications: WB

Reactivity: Human, Mouse

Conjugate: HRP