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Primary Antibodies

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Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program (badge number 29749). Lane 1: A549 cell extract Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (bs-0588R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0588R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:300 followed by conjugation to a secondary antibody and staining.
Lane 1: Mouse Testis lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Testis lysates; Lane 4: Rat Cerebrum lysates probed with GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0654R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Unconjugated

Catalog #1: bs-0654R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Biotin

Catalog #1: bs-0654R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: HRP

Catalog #1: bs-0654R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: FITC

Catalog #1: bs-0654R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Cy3

Catalog #1: bs-0654R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Cy5

Catalog #1: bs-0654R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Cy7

Catalog #1: bs-0890R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Unconjugated

Catalog #1: bs-0890R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Pig, Tags

Conjugate: HRP

Catalog #1: bs-0890R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Pig, Tags

Conjugate: Biotin

Catalog #1: bs-0890R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy3

Catalog #1: bs-0890R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy7

Catalog #1: bs-0890R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: FITC

Catalog #1: bs-0890R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy5

Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti-NGN3\/Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval with 0.4% pepsin at 37\u00b0C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Liver; Probed Neurogenin 3, unconjugated (bs-0922R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Breast Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurogenin 3 Polyclonal Antibody, Unconjugated (bs-0922R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-0922R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0922R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-0922R-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-0922R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-0922R-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-0922R-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-0922R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Formalin-fixed and paraffin embedded rat aortic cavity labeled with Rabbit Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat intestine tissue labeled with Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: GM-CSF+Trx\/His-tag fusion protein\uff1bLane 2: GM-CSF fusion protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-0999R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0999R

Applications: WB

Reactivity: Pig

Conjugate: Unconjugated

Catalog #1: bs-10125R

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-10125R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-10125R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-10130R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-10130R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-10130R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-10130R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-10130R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-10130R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-10130R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti CMKLR1 Polyclonal Antibody, Unconjugated (bs-12478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Huvec (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMKLR1 Antibody (bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Liver tissue lysates probed with CMKLR1 Polyclonal Antibody, Unconjugated (bs-10185R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-10185R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-10185R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-10185R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-10185R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-10185R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-10185R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-10185R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD

Catalog #1: bs-1032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-1032R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-1032R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-1032R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-1032R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-1032R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-1032R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-10498R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Pig

Conjugate: Unconjugated