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Primary Antibodies

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Catalog #1: bsm-33006M-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded dog bladder labeled with Anti-Alpha-SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: dog bladder tissue labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300, followed by conjugation to the secondary antibody.
Mouse Small Intestine labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated(bs-0189R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C
NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with alpha Actin Polyclonal Antibody(bs-0189R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0189R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Unconjugated

Antigen: bs-0078P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Human liver lysates probed with Anti TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 17kD, 26kD. Observed band size:26kD.

Catalog #1: bs-0078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-0078R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Biotin

Catalog #1: bs-0078R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: HRP

Catalog #1: bs-0078R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Cy3

Catalog #1: bs-0078R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Cy5

Catalog #1: bs-0078R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-0078R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: Cy7

Catalog #1: bs-0078R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Others

Conjugate: FITC

Catalog #1: bs-0636R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-0879R

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse placenta lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0718R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit, Goat, Others

Conjugate: Unconjugated

Myc-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41230P) probed with c-Myc tag Polyclonal Antibody, Unconjugated (bs-0842R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: E.coli Lysate lysates probed with c-Myc tag Polyclonal Antibody, Unconjugated (bs-0842R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0842R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-0842R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #1: bs-0842R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Tags, Others

Conjugate: HRP

Catalog #1: bs-0842R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: Cy3

Catalog #1: bs-0842R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: Cy5

Catalog #1: bs-0842R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: Cy5.5

Catalog #1: bs-0842R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: Cy7

Catalog #1: bs-0842R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags, Others

Conjugate: FITC

Catalog #1: bs-10011R-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Occludin Polyclonal Antibody(bs-10011R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10011R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Unconjugated

Catalog #1: bs-10011R-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: HRP

Catalog #1: bs-10011R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy3

Catalog #1: bs-10011R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy5.5

Catalog #1: bs-10011R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy5

Catalog #1: bs-10011R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: FITC

Catalog #1: bs-10011R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others

Conjugate: Cy7

Catalog #1: bs-10172r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:200 overnight at 4\u00b0C, DAB staining.
Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-10172R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-10172r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-10172r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bs-10172r-cy3-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-10172r-cy5-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-10172r-cy7-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bs-10172r-cy55-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5

Catalog #1: bs-10172r-fitc-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bs-3417R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-3417R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Others

Conjugate: HRP

Catalog #1: bs-3417R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Cy3

Catalog #1: bs-3417R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Cy5

Catalog #1: bs-3417R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Cy5.5

Catalog #1: bs-3417R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: FITC

Catalog #1: bs-3417R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Cy7

Catalog #1: bs-3417R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Others

Conjugate: Biotin

HeLa cells were fixed with 70% ice-cold methanol overnight at 4\u2103\uff0cpermeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H3 (tri methyl K37) Antibody(bs-3768R\r\n) at 1\uff1a50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K37) Polyclonal Antibody, Unconjugated (bs-3768R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Siha cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U-2OS cell lysates; Lane 4: Human Jurkat cell lysates probed with Histone H3 Polyclonal Antibody, Unconjugated (bs-3768R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3768R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-3768R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Others

Conjugate: HRP

Catalog #1: bs-3768R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Others

Conjugate: Biotin