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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nJAK1 Monoclonal Antibody, Unconjugated (bsm-33268M) at 1:400 overnight at 4\u00b0C, DAB staining.
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK1 Monoclonal Antibody(bsm-33267M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33268M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33268M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33268M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33268M-Cy3

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33268M-Cy5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33268M-Cy5.5

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33264M-Cy3

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33268M-Cy7

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33268M-FITC

Applications: IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33283M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human HT-1080 cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human SH-SY5Y cell lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33283M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33283M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33283M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33283M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33283M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33283M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33283M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33283M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33283M-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (bsm-33284M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33284M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33284M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33284M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33284M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33284M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33284M-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33284M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33284M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33301M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33301M-Cy5.5

Applications: IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (Tyr705) Monoclonal Antibody, Unconjugated (bsm-33301M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (Tyr705) Monoclonal Antibody, Unconjugated (bsm-33301M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (Tyr705) Monoclonal Antibody, Unconjugated (bsm-33301M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (Tyr705) Monoclonal Antibody, Unconjugated (bsm-33301M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33301M

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33301M-Cy3

Applications: IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33301M-Cy5

Applications: IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33301M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33301M-Cy7

Applications: IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33301M-FITC

Applications: IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33400M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33400M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33400M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33400M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33400M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33400M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33400M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Myoglobin (3A7) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Myoglobin) Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Rat Muscle tissue lysates probed with Myoglobin Monoclonal Antibody, Unconjugated (bsm-41107M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41107M

Applications: IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41107M-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-41107M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-41107M-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-41107M-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-41107M-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-41107M-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-41107M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP