New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies

Collection Menu
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-CDK4 Polyclonal Antibody (bs-0633R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti CDK4 Polyclonal Antibody, Unconjugated (bs-0633R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 33kD. Observed band size: 33kD.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CDK4 Polyclonal Antibody, Unconjugated (bs-0633R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDK4 Polyclonal Antibody, Unconjugated (bs-0633R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0633R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-0633R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Biotin

Catalog #1: bs-0633R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: HRP

Catalog #1: bs-0633R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy3

Catalog #1: bs-0633R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5

Catalog #1: bs-0635R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0633R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-0633R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy7

Catalog #1: bs-0635R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0635R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0635R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0635R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0635R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0635R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P); Lane 2: Control (-) E.coli lysates probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GFP-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-33009P) probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000~10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GFP-tagged fusion protein probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:20000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0639R

Applications: WB, IF

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-0640R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti TIGAR Polyclonal Antibody, Unconjugated (bs-0640R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti TIGAR Polyclonal Antibody, Unconjugated (bs-0640R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-0640R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0640R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-0640R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0640R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-0640R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0640R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-0641R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: Biotin

Catalog #1: bs-0641R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Integrin alpha 4\/CD49d\/Integrin \u03b14 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with Integrin alpha 4\/CD49d Antibody, Unconjugated (bs-0641R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
U-937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4\/CD49dAntibody( bs-0641R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Polyclonal Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0641R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0641R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy3

Catalog #1: bs-0642R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0641R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy5

Catalog #1: bs-0641R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy7

Catalog #1: bs-0641R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: FITC

Catalog #1: bs-0641R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rabbit

Conjugate: PE

Catalog #1: bs-0642R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0642R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0642R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0642R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0642R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0642R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-0651R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
MCF-7 cells were stained with Connexin 43 Polyclonal Antibody, Unconjugated(bs-0651R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Hela cells probed with Connexin 43 Polyclonal Antibody, unconjugated (bs-0651R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0651R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0651R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-0651R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-0651R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0651R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0651R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0651R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0651r-pe-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0652R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0652R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0652R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP