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Primary Antibodies

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Catalog #1: bs-0456R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0456R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0456R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0463R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0463R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-0463R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-0466R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine, Sheep, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-0463R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-0467R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0463R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-0467R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-0467R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-0467R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 25kD. Observed band size:25kD.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0471R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-0467R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0474R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0474R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0474R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0474R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0474R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Formalin-fixed and paraffin embedded rat lung labeled with Anti- VSIG4 Polyclonal Antibody, Unconjugated (bs-0479R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse lung and placenta lysates probed with VSIG4 Polyclonal Antibody, unconjugated (bs-0479R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-0479R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human kidney tissue labeled with Rabbit Anti- IFN-gamma \\nPolyclonal Antibody, Unconjugated (bs-0481R) at 1:200 followed by conjugation to the secondary \\nantibody and DAB staining
HL-60 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0481R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0481R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0481R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0481R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0481R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0483R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-0483R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy3

Catalog #1: bs-0483R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy7

Catalog #1: bs-0483R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Cy5

Catalog #1: bs-0483R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: FITC

Catalog #1: bs-0484R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0484R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bs-0484R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy3

Catalog #1: bs-0484R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bs-0484R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: FITC

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse heart lysates probed with Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 20\/48kD. Observed band size:20kD.

Catalog #1: bs-0488R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0488R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0488R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0488R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0488R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10\u03bcM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.

Catalog #1: bs-0489R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-0489R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Cy7

Catalog #1: bs-0489R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Cy3

Catalog #1: bs-0489R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Cy5

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PPP4C Polyclonal Antibody(bs-0490R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0490R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0489R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: FITC