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Primary Antibodies

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Lane 1: mouse brain lysates Lane 2: mouse thyroid lysates probed with Anti TTF-2\/FOXE1 Polyclonal Antibody, Unconjugated (bs-0446R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 41kD. Observed band size: 41kD.
Mouse kidney lysates probed with FOXE1 Polyclonal Antibody, Unconjugated (bs-0446R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0446R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0446R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0446R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat thyroid gland labeled with Anti-NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti NIS Polyclonal Antibody, Unconjugated (bs-0448R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 65kD. Observed band size:65kD.
Formalin-fixed and paraffin-embedded human thyroid labeled with Rabbit Anti-NIS Polyclonal Antibody, Unconjugated(bs-448R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0448R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-TSLP Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0449R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0452R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0451R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0452R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0452R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0456R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0456R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0456R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-TSHR Polyclonal Antibody (bs-0460R)at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse thymocytes probed with Rabbit Anti-TSHR Polyclonal Antibody, Unconjugated (bs-0460R) (green) at 1:100 for 60 minutes followed by a FITC conjugated secondary compared to control cells (blue).

Catalog #1: bs-0460R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0460R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0460R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0463R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0463R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-0463R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-0466R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine, Sheep, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-0467R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0467R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-0467R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 25kD. Observed band size:25kD.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0471R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-0473R

Applications: ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0473R-HRP

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0473R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0474R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0474R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0474R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat lung labeled with Anti- VSIG4 Polyclonal Antibody, Unconjugated (bs-0479R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse lung and placenta lysates probed with VSIG4 Polyclonal Antibody, unconjugated (bs-0479R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-0479R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human kidney tissue labeled with Rabbit Anti- IFN-gamma \\nPolyclonal Antibody, Unconjugated (bs-0481R) at 1:200 followed by conjugation to the secondary \\nantibody and DAB staining
HL-60 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0481R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0481R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0481R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0483R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: HRP

Catalog #1: bs-0483R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-0483R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bs-0484R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-0484R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0484R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-0485R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti EphA2\/Eph receptor A2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-EphA2\/Eph receptor A2 Polyclonal Antibody, Unconjugated(bs-0485R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-EphA2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:100 followed by conjugation to the secondary antibody and DAB staining\\n
NIH\/3T3 cell lysates probed with EphA2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0485R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0485R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse heart lysates probed with Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 20\/48kD. Observed band size:20kD.

Catalog #1: bs-0488R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0488R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0488R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0489R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Others

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10\u03bcM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.

Catalog #1: bs-0489R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Unconjugated