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Vimentin Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Pig
  • Others
  • Monkey

Predicted Reactivity

  • Cow
  • Chicken
  • Goat
Overview
Catalog # bs-0756R-Cy5
Product Name Vimentin Polyclonal Antibody, Cy5 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity KO-Validated
Reactivity Human, Mouse, Rat, Bovine, Pig, Others, Monkey
Predicted Reactivity Cow, Chicken, Goat
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Vimentin
Immunogen Range 371-466/466
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7431
Swiss Prot P08670
Subcellular location Cytoplasm, Extracellular matrix
Synonyms HEL113; CTRCT3; Vimentin; VIM
Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200