7-Amino-4-trifluoromethylcoumarin
| Overview | |
| Catalog # | bs-75807c-1g |
| Product Name | 7-Amino-4-trifluoromethylcoumarin |
| Specifications | |
| Storage Buffer | Solid |
| Storage Condition | Stable for 2 years after receipt when stored at RT. |
| Target | |
| Product Information | CAS Number: 53518-15-3 Molecular formula: C10H6F3NO2 Molecular weight: 229.16 Purity: >99% (GC) Appearance: Yellow solid. Solubility: Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water. InChiKey: JBNOVHJXQSHGRL-UHFFFAOYSA-N SMILES: NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1 |
| Description | 7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: lambdaex 376nm, lambdaem 482nm, Quantum yield 0.531. |
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