Human HP1 alpha Ready-To-Use IHC Kit
Human HP1 alpha Ready-To-Use IHC Kit
Cat. No.: IHC0116HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
Synonyms
Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha.
Materials Supplied
Number | Component | 50T | Concentration | Storage |
---|---|---|---|---|
1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
4 | Blocking Buffer | 3ml | RTU | 2-8°C |
5 | Primary Antibody (Human HP1 alpha Mouse mAb) | 6ml | RTU | 2-8°C |
6 | Secondary Antibody (HRP-Goat anti-Mouse IgG pAb) | 6ml | RTU | 2-8°C |
7 | Chromogen Component A | 0.3ml | RTU | -20°C |
8 | Chromogen Component B | 0.3ml | RTU | -20°C |
9 | Counter Staining Reagent | 5ml | RTU | RT |
10 | Differentiation Reagent | 6ml | RTU | RT |
11 | Mounting Media | 5ml | RTU | RT |
12 | Control slide (Human prostate tumor) | 1 slide | RTU | RT |
13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
Please cite this product as " IHC0116H, Bioss Antibodies".
Citation example: “Human tissue sections using Human HP1 alpha IHC Kit (IHC0116H, Bioss Antibodies) were stained for HP1 alpha according to the manufacturer's instructions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.