Overview |
bs-9673R-Cy3 |
MEL18 Polyclonal Antibody, Cy3 Conjugated |
WB, IF(IHC-P) |
Human, Mouse, Rat |
Specifications |
Cy3 |
Rabbit |
KLH conjugated synthetic peptide derived from human MEL18 |
145-245/344 |
Polyclonal |
IgG |
1ug/ul |
Purified by Protein A. |
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
Target |
7703 |
P35227 |
Nucleus |
MEL-18; RNF110; ZNF144; Polycomb group RING finger protein 2; DNA-binding protein Mel-18; RING finger protein 110; Zinc finger protein 144; PCGF2; MEL18 |
Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex (By similarity). |
Application Dilution |
WB |
1:300-5000 |
IF(IHC-P) |
1:50-200 |