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Cdc25B (Ser149) Polyclonal Antibody, APC-Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-8445R-APC-Cy5
Product Name Cdc25B (Ser149) Polyclonal Antibody, APC-Cy5 Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation APC-Cy5
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149
Modification Site Ser149
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 12531
Swiss Prot P30306
Subcellular location Cytoplasm
Synonyms AI604853; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; Cdc25b; Cdc25m2
Background Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200