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B-Raf (Ser365) Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3013R-HRP
Product Name B-Raf (Ser365) Polyclonal Antibody, HRP Conjugated
Applications ELISA, IHC-P, IHC-F
Specificity This phophorylation site is homologous to that of Ser348 in Mouse and Ser347 in Rat.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human B Raf around the phosphorylation site of Ser365
Modification Site Ser365
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 673
Swiss Prot P15056
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms NS7; BRAF1; RAFB1; B-RAF1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF
Background Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500