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Ki-67 Polyclonal Antibody, AbBy Fluor® 405 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-23102R-BF405
Product Name Ki-67 Polyclonal Antibody, AbBy Fluor® 405 Conjugated
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation AbBy Fluor® 405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Ki-67
Immunogen Range 2901-3000/3256
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4288
Swiss Prot P46013
Subcellular location Nucleus
Synonyms Proliferation marker protein Ki-67; MKI67; Antigen identified by monoclonal antibody Ki-67; Antigen KI67
Background Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200