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BNIP3 (Ser95) Polyclonal Antibody, AbBy Fluor® 647 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-12875R-BF647
Product Name BNIP3 (Ser95) Polyclonal Antibody, AbBy Fluor® 647 Conjugated
Applications IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human
Predicted Reactivity Dog, Cow, Sheep, Horse
Specifications
Conjugation AbBy Fluor® 647
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human BNIP3 around the phosphorylation site of Ser95
Modification Site Ser95
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 664
Subcellular location Cytoplasm, Cell membrane
Synonyms BNIP3 phospho S95; p-BNIP3 phospho S95; BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3; BNIP3_HUMAN; NIP 3; NIP3.
Background The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200