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Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated (bs-2067R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: SW480 cell lysates; Lane 2: NIH\/3T3 cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-2067R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2067R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric tissue labeled with Rabbit Anti-NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse lung lysates probed with Rabbit Anti-NFkB p105\/p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1194R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti IGFBP6 Polyclonal Antibody, Unconjugated (bs-4064R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 mouse intestine lysates probed with Anti IGFBP6 Polyclonal Antibody, Unconjugated (bs-4064R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 23kD. Observed band size:23kD.\\n

Catalog #1: bs-4064R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 human colon carcinoma lysates L2 human gastric carcinoma lysates probed with Anti CHK2 Polyclonal Antibody, Unconjugated (bs-1391R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 61kD. Observed band size: 61kD
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHK2 Polyclonal Antibody(bs-1391R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1391R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti GRM1 Polyclonal Antibody,Unconjugated (bs-1803R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma tissue labeled with Anti GRM1 Polyclonal Antibody,Unconjugated (bs1803R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse brain lysates Lane 2: mouse embryo lysates probed with Anti GRM1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 129kD. Observed band size: 129kD.
SK-N-MC cells probed with Rabbit Anti-Group I mGLUR Polyclonal Antibody (bs-1803R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.

Catalog #1: bs-1803R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5\/MAP2K5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated (bs-4124R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4124R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK alpha Polyclonal Antibody, Unconjugated (bs-2907R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK alpha Polyclonal Antibody, Unconjugated (bs-2907R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK alpha Polyclonal Antibody, Unconjugated (bs-2907R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK alpha Polyclonal Antibody, Unconjugated (bs-2907R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2907R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Ubiquityl Histone H2A.X (Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti Ubiquityl Histone H2A.X (Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 16kD. Observed band size: 16kD.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2A.X (Ubiquityl Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H2A.X (Ubiquityl Lys119) Antibody(bs-8571R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-8571R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse intestinal lysates Lane 2: mouse testicular lysates probed with Rabbit Anti-Cdc25C Polyclonal Antibody, Unconjugated (bs-9597R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-Cdc25C Polyclonal Antibody, Unconjugated (bs-9597R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cdc25C) polyclonal Antibody, Unconjugated (bs-9597R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cdc25C Polyclonal Antibody(bs-9597R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-9597R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Liver; Lane 2: Mouse Ovary; 40ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Raw264.7 Cells; Lane 2: Human Hela Cells; 30ug loaded in each lane; Probed with TLR5 Polyclonal Antibody, unconjugated (bs-1197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-1197R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung cancer tissue labeled with Anti-RAP1A Polyclonal Antibody, Unconjugated (bs-1504R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-RAP1A Polyclonal Antibody, Unconjugated (bs-1504R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
HepG2 cell lysates probed with Anti-RAP1A Polyclonal Antibody, Unconjugated (bs-1504R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C

Catalog #1: bs-1504R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human flat moss tinea tissue labeled with Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (bs-1148R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat ovary labeled with Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (bs-1148R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Cyclin D2 Polyclonal Antibody (bs-1148R) at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-Cyclin D2 Polyclonal Antibody (bs-1148R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-1148R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2093R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Mouse, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Synaptotagmin 1\/SYT1 Polyclonal Antibody, Unconjugated (bs-4172R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat heart, L2 rat brain lysates probed (bs-4172R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 46kDa.\\n
Lane 1: Human HepG2 cell lysates; Lane 2: Human A673 cell lysates; Lane 3: Human HeLa cell lysates probed with Synaptotagmin 1 Polyclonal Antibody, Unconjugated (bs-4172R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4172R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti-XIAP\/BIRC4 Polyclonal Antibody, Unconjugated (bs-1281R) followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-XIAP Polyclonal Antibody (bs-1281R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-XIAP Polyclonal Antibody (bs-1281R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-1281R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (bs-1703R) followed by conjugation to the secondary antibody and DAB staining
Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf\/Raf1 Polyclonal Antibody (bs-1703R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Catalog #1: bs-1703R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3503R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Rabbit Anti phospho-Crkl(Tyr251) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates L2 mouse intestine lysates probed with Anti phospho-Crkl(Tyr251) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 33kD. Observed band size:33kD.\\n
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Crkl) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Crkl) Polyclonal Antibody, Unconjugated (bs-3738R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3738R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Nucleophosmin Polyclonal Antibody, Unconjugated (bs-4757R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysate L2 rat brain lysates probed with Anti Nucleophosmin Polyclonal Antibody, Unconjugated (bs-4757R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 33kD. Observed band size: 33kD
Formalin-fixed and paraffin embedded human kidney labeled with Anti-Nucleophosmin Polyclonal Antibody, Unconjugated (bs-4757R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SH-SY5Y cell lysates probed with Nucleophosmin Polyclonal Antibody, Unconjugated (bs-4757R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4757R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (bs-0623R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0623r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Unconjugated

U251 cells (human glioma cells) were probed with Rabbit Anti-CASPR\/Neurexin4 Polyclonal Antibody, Unconjugated(bs-11128R) 1:200, followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CASPR\/Neurexin4) Polyclonal Antibody, Unconjugated (bs-11128R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-11128R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (bs-1433R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. \\n
Image provided by the Independent Validation Program (badge number 29760). Lane 1: HeLa cell extract, Lane 2: NIH\/3T3 cell extracts, Lane 3: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1433R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei\\n
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3010R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated