WNT2 Polyclonal Antibody

$Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.$

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.

$Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.$

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse

Overview
Catalog #	bs-6133R
Product Name	WNT2 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human WNT2
Immunogen Range	221-320/360
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7472
Swiss Prot	P09544
Subcellular location	Secreted
Synonyms	IRP; INT1L1; Protein Wnt-2; Int-1-like protein 1; Int-1-related protein; WNT2
Background	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Application Dilution
WB	1:300-5000
ELISA	1:5000-10000