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ATG1(Ser556) Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
  • Horse
Overview
Catalog # bs-3464R-HRP
Product Name ATG1(Ser556) Polyclonal Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig, Horse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556
Modification Site Ser556
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8408
Swiss Prot O75385
Subcellular location Cytoplasm
Synonyms ATG1; ATG1A; UNC51; hATG1; Unc51.1; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1; ULK1; KIAA0722
Background Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500