New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

C-Raf (N-terminal region) Antibody

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70641M
Product Name C-Raf (N-terminal region) Antibody
Applications WB
Specificity This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.
Clonality Monoclonal
Clone # M208
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P04049
Synonyms Raf1, CRaf
Background The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation
Application Dilution
WB 1:300-5000