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His (C-terminal) Tag Antibody

Applications

  • WB
  • FCM
  • IP
  • IHC
  • ICC

Reactivity

  • Mouse
  • Rat
  • Bacteria
  • Tags
Overview
Catalog # bsm-70513M
Product Name His (C-terminal) Tag Antibody
Applications WB, FCM, IP, IHC, ICC
Specificity Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunoprecipitation, immunocytochemistry, flow cytometry, and ELISA capture.
Reactivity Mouse, Rat, Bacteria, Tags
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.
Clonality Monoclonal
Clone # M050
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot N/A
Synonyms His Tag, 6x His Tag, HHHHHH
Background Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IP 1-2ug
IHC
ICC 1:100-500