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EphA2 (Extracellular region) M060 Antibody

Applications

  • WB
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70480M
Product Name EphA2 (Extracellular region) M060 Antibody
Applications WB, ICC
Specificity Clone M060 mouse monoclonal antibody detects a 125kDa* protein on SDS-PAGE "Native" immunoblots of human NCI-H2052 and MDA-MB-231 carcinomas, as well as the EphA2 C-terminal 60 kDa fragement in human A431 and A549. The antibody detects native EphA2, as well as aldehyde-fixed EphA2. The antibody works for Native western blot, ELISA capture, and immunocytochemistry.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M060) was generated from a recombinant protein that included the extracellular region of human EphA2 protein.
Clonality Monoclonal
Clone # M060
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P29317
Synonyms EphA2, P29317, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, Ephrin type-A receptor 2
Background The Eph family of receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. Ephrin type-A receptor 2 (EphA2), also known as epithelial cell kinase (Eck), binds the ephrin A1 (EFNA1) ligand, and has roles in neuronal development and repair, as well as carcinogenesis. EphA2 receptor has an N-terminal ligand-binding domain followed by a cysteine-rich domain with an epidermal growth factor-like motif and two fibronectin type-III repeats in the extracellular region, and a sterile alpha motif (SAM), and a PDZ domain-binding motif in the intracellular region. EphA2 is expressed in many types of cancers, including breast, colon, bladder, gastric, and glioblastoma. In bladder cancers, EphA2 may be activated by progranulin leading to phosphorylation at Ser-897 and bladder tumorigenesis. EphA2 may be an important therapeutic target and biomarker for several types of cancer.
Application Dilution
WB 1:300-5000
ICC 1:100-500