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Connexin-43 (C-terminal region) Antibody

Applications

  • WB
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
  • Chicken
Overview
Catalog # bsm-70425M
Product Name Connexin-43 (C-terminal region) Antibody
Applications WB, ICC
Specificity The antibody detects a 43 kDa* protein corresponding to the apparent molecular mass of connexin-43 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells.
Reactivity Human, Mouse, Rat, Chicken
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43.
Clonality Monoclonal
Clone # M496
Isotype IgG1
Purification Antigen Affinity purification
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P17302
Synonyms Gap junction alpha-1 43 GJA1 CXA1
Background Connexin-43 (Cx43, CXA1, Gap Junction α1) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels that cluster together to form gap junctions. Gap junction communication is critical for cell to cell communication during development and regulation of cell growth. Phosphorylation of connexin-43 is important in regulating both the assembly and the function of gap junctions. PKC phosphorylates Ser-368 in connexin-43 after activation of cells with phorbol esters. This phosphorylation event decreases cell-to-cell communication. In addition, the tyrosine kinase, c-Src, can interact with and phosphorylate Tyr-265 in connexin-43 leading to inhibition of gap junction communication. Thus, connexin-43 phosphorylation may be an important mode for regulating gap junctional communication.
Application Dilution
WB 1:300-5000
ICC 1:100-500