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CRISPR Cas9 Antibody

Applications

  • WB
  • IHC
  • ICC

Reactivity

  • Others
Overview
Catalog # bsm-70054M
Product Name CRISPR Cas9 Antibody
Applications WB, IHC, ICC
Specificity Specific for the Staphylococcus aureus Cas9 protein.
Reactivity Others
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Clonality Monoclonal
Clone # 6F7
Isotype IgG1
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer PBS + 50% glycerol and 5 mm NaN3
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Synonyms Cas9 antibody, CRISPR-associated endonuclease Cas9/Csn1 antibody, CRISPR-Cas9/Csn1 antibody, csn1 antibody, SpyCas9 antibody
Background CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).
Application Dilution
WB 1:300-5000
IHC
ICC 1:100-500