X-Gal
Overview | |
Catalog # | bs-75002c-50mg |
Product Name | X-Gal |
Specifications | |
Storage Buffer | Powder |
Storage Condition | Stable for 3 years after receipt when stored at -20°C. |
Target | |
Product Information | CAS Number: 7240-90-6 Molecular formula: C14H15BrClNO6 Molecular weight: 408.6 Purity: >99% (HPLC) Appearance: White to off-white powder. Clear, colourless to light yellow in solution. Solubility: Soluble in dimethyl formamide. InChiKey: OPIFSICVWOWJMJ-LMNFNBGQSA-N SMILES: OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O |
Description | Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. bs-75001C) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples. |