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Catalog #1: bs-12958R-PerCP-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP-Cy7

Catalog #1: bs-12958R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: RBITC

Catalog #1: bs-12959R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Mouse pancreas lysates probed with Actin (Tyr53) Polyclonal Antibody, Unconjugated (bs-12581R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Actin (Tyr53) Polyclonal Antibody(bs-12581R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-12581R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12581R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12447R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3002R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3002R-BF350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-3002R-BF405

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-3002R-BF488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-3002R-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-3002R-BF594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-3002R-BF647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-3002R-BF680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bs-3002R-BF750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bs-3002R-APC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-3002R-APC-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy5

Catalog #1: bs-3002R-APC-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-3002R-APC-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy7

Catalog #1: bs-3002R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-3002R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-3002R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-3002R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-3002R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-3002R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-3002R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-3002R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP

Catalog #1: bs-3002R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3002R-PerCP-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-3002R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: RBITC

Catalog #1: bs-3072R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70330M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33281M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bsm-33281M-BF350

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-33281M-BF405

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-33281M-BF488

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-33281M-BF555

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-33281M-BF594

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-33281M-BF647

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-33281M-BF680

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-33281M-BF750

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-33281M-APC

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC

Catalog #1: bsm-33281M-APC-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC-Cy5

Catalog #1: bsm-33281M-APC-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC-Cy5.5

Catalog #1: bsm-33281M-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC-Cy7

Catalog #1: bsm-33281M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bsm-33281M-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bsm-33281M-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bsm-33281M-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5