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Catalog #1: bsm-72052M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72054M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72055M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70422T

Applications: WB, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72083M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: Human Brain tissue lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:8000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Brain tissue lysates probed with CRMP2 Monoclonal Antibody, Unconjugated (bsm-51453M) at 1:12800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51453M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates; probed with DDR1 (4C4) Monoclonal Antibody, unconjugated (bsm-51295M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DDR1 (4C4) Monoclonal Antibody (bsm-51295M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51295M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: human skeletal muscle lysates; Lane 3: human brain Cell lysates; probed with DENR (2C9) Polyclonal Antibody, unconjugated (bsm-51318M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using DENR (2C9) Monoclonal Antibody bsm-51318M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51318M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72127M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70459M

Applications: WB, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72116M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72117M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72118M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72119M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72120M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72121M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: A431; Lane 3: HeLa; Lane 4: HepG2; Lane 5: NIH\/3T3; Lane 6: mouse stomach; Lane 7: mouse liver lysate at 35 \u00b5g per lane. probed with bsm-51216M ENO1 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ENO1 (7C4) Monoclonal Antibody (bsm-51216M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51216M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-72133M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72134M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using ETS1 (1C1) Monoclonal Antibody (green) unconjugated bsm-51344M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51344M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70085M

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bsm-72149M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-70092M

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-72155M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72157M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: human brain lysate; Lane 2: human cerebellum lysate at 20 \u00b5g per lane, probed with bsm-51047M GFAP (183CT3.1.5) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: MCF-7 Cell lysates; probed with GFAP (183CT3.1.5) Monoclonal Antibody, unconjugated (bsm-51047M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51047M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-72160M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72162M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-51023R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K4me1 Monoclonal Antibody (bsm-53013M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the molecular weight marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me1 (34A1) Monoclonal Antibody (bsm-53013M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53013M

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51013R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4un antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M). The wells were coated with peptides containing the unmodified H3K4 region as well as the mono-, di- and trimethylated H3K4. Figure 2 shows a high specificity of the antibody for the unmodified peptide.
ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4un (Cat. No. bsm-53012M) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53012M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51012R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72181M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72186M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72193M

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72213M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72228M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72233M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72240M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51163M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72251M

Applications: WB, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72257M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72258M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72260M

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72269M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72272M

Applications: ELISA

Reactivity:

Conjugate: Unconjugated