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HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human kidney section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
NIH\/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52015R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-70637M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-60249c-2-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82971c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6772R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with A1CF Polyclonal Antibody, Unconjugated (bs-6773R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with A1CF Polyclonal Antibody, Unconjugated (bs-6773R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6773R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-75842c-25mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6513R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse Liver lysates probed with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-11381R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6834c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7588R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-25228R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11382R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7134c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60020c-5-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-80253C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82241c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6910c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-80187C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82179c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6893c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82961c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82687c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60143c-5-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11383R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0094R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AADAC Polyclonal Antibody, Unconjugated (bs-11384R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AADAC Polyclonal Antibody, Unconjugated (bs-11384R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-11384R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5162R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11385R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11386R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11387R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60434c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7133c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1507R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-80022C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82021c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11388R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11448R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-AANAT Polyclonal Antibody, Unconjugated (bs-3914R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-AANAT Polyclonal Antibody, Unconjugated (bs-3914R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C
Antigen: bs-3914P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AANAT Polyclonal Antibody, Unconjugated (bs-3914R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3914R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-AARE Polyclonal Antibody, Unconjugated (bs-5983R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Hela cell lysates;Lane 2: Hl60 cell lysates;Lane 3: U2OS cell lysates; Lane 4: U87mg cell lysates probed with AARE Polyclonal Antibody, Unconjugated (bs-5983R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5983R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1603R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11389R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-11390R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11391R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: 293T, 2: HeLa, 3: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 63 kDa\r\nObserved MW: 80 kDa\r\n
Cell line: HeLa\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nCounter stain: Tubulin (Red)\r\nComment: Color green is the positive signal for PTM-6030\r\n
Cell line: HEK-293 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4\u00b0C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for bsm-60590R\r\n

Catalog #1: bsm-60590R

Applications: WB, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1229R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7103c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7132c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated