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Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ET-1 Polyclonal Antibody, Unconjugated (bs-0202R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates probed with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0202R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12866R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: Mouse Heart; Lane 2: Human Skin; Probed with Biglycan (4A8) Monoclonal Antibody (bsm-54293R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with Biglycan (4A8) Monoclonal Antibody (bsm-54293R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Biglycan (4A8) Monoclonal Antibody (bsm-54293R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with Biglycan (4A8) Monoclonal Antibody (bsm-54293R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54293R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed (4%) Human Endothelial Cells were blocked with normal goat serum for 20mins at 37\u2103. The cells were then labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:50, overnight at 4\u00b0C. Secondary antibody incubation was performed with Goat Anti-Rabbit IgG, Rhodamine B conjugated antibody (bs-0295G-RBITC) at a 1:200 dilution for 40 minutes at 37\u00b0C. DAPI (5ug\/ml; blue) was used to stain the cell nuclei
Formalin-fixed and paraffin-embedded Human Lung Carcinoma labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:200 followed by secondary antibody, DAB and counterstaining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse Bone probed with Biglycan Polyclonal Antibody (bs-7552R) at 1:300, overnight at 4˚C. Followed by incubation with a conjugated secondary antibody.

Catalog #1: bs-7552R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-82962c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3784R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-23762R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-75111c-250ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Lane 1: HepG2 lysates probed with Bile Acid Receptor NR1H4 Antibody (bs-12867R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.\r\n
Mouse liver lysates probed with Bile Acid Receptor NR1H4 Polyclonal Antibody, Unconjugated (bs-12867R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Rat Liver tissue lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human MCF-7 cell lysates probed with NR1H4 Polyclonal Antibody, Unconjugated (bs-12867R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with NR1H4 Antibody(bs-12867R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-12867R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-5042R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: Raji cell ; Lane 2: Jurkat cell; Lane 3: A431 cell; Probed with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Bim (42C9) Monoclonal Antibody (bsm-52409R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52409R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60641R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-25552R

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1488R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1485R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9727R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7739R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-83020c-5-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75917c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-83117c-1-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75934c-100mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75933c-25mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60088c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-80476C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82459c-100-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75345c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6787c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75399c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75346c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75401c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6835c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75411c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75400c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75357c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75403c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75402c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6816c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75398c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-71147R

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bs-71148R

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bs-75926c-50mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82926c-100-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12869R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75382c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75409c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7121c-2mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60216c-2-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated