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Catalog #1: bs-75436c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60107c-2-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: AK2 knockout (KO) 293T cell lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL-60 cell lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Liver lysates probed with AK2 Polyclonal Antibody, Unconjugated (bs-55004R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55004R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7903R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-18605R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKAP12 Polyclonal Antibody, Unconjugated (bs-1381R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKAP12 Polyclonal Antibody, Unconjugated (bs-1381R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKAP12\/Gravin Polyclonal Antibody, Unconjugated (bs-1381R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-1381R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51633M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: Jurkat Cells; Lane 2: A549 Cells; Probed with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52545R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with AKAP2 Polyclonal Antibody, Unconjugated (bs-9028R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKAP2 Polyclonal Antibody, Unconjugated (bs-9028R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKAP2 Polyclonal Antibody, Unconjugated (bs-9028R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-9028R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6980R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-7904R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11999R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1473R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9532R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4287R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKIRIN1 Polyclonal Antibody, Unconjugated (bs-9081R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKIRIN1 Polyclonal Antibody, Unconjugated (bs-9081R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9081R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9082R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse pancreas lysates Lane 2: mouse brain lysates probed with Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 36kD. Observed band size: 36kD.
Mouse liver lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver cell lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7953R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55005R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) followed by conjugation to the secondary antibody
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Adrenal gland tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human A549 cell lysates; Lane 7: Human U87MG cell lysates probed with AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2405R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55006R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11401R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-70330M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT (Ser473)(9H11) Monoclonal Antibody, Unconjugated (bsm-33281M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33281M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-70331R

Applications: WB

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-70012R

Applications: WB

Reactivity: Drosophila

Conjugate: Unconjugated

Catalog #1: bs-3043R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33282M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6708c-10mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

MCF-7 lysates probed with AKT1 (11F2) Monoclonal Antibody (bsm-52010R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Lung lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Cerebellum lysates; Lane 7: Rat Thymus lysates; Lane 8: Rat Heart lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human U937 cell lysates; Lane 12: Human A549 cell lysates probed with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (11F2) Monoclonal Antibody, Unconjugated (bsm-52010R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52010R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51238M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (Ser473 + Tyr474) Polyclonal Antibody, Unconjugated (bs-10134R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1 (Ser473 + Tyr474) Polyclonal Antibody, Unconjugated (bs-10134R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-10134R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12456R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF7, 2: HeLa, 3: A549\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 56 kDa\r\nObserved MW: 56 kDa
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R
Tissue: Human colon carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R
Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60670R

Catalog #1: bsm-60670R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF-7, 2: HeLa, 3: Mouse brain, 4: \r\nRat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 56 kDa\r\nObserved MW: 56 kDa
Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60702R
Tissue: Rat cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60702R

Catalog #1: bsm-60702R

Applications: WB, IHC-P, IHC-F

Reactivity:

Conjugate: Unconjugated

Lane 1: NIH\/3T3 treated with PDGF; Lane 2: NIH\/3T3 untreatedlysates probed with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody. The bottom panel is antibody staining using an unphosphorylated AKT1 antibody.
3T3 cells were stained with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse lung section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
MCF7 cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1 (Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52130R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33278M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1(Ser129) Polyclonal Antibody, Unconjugated (bs-5188R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1(Ser129) Polyclonal Antibody, Unconjugated (bs-5188R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5188R

Applications: ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10996R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

MCF-7 Cell lysates; probed with AKT1(T450) (4A3) Monoclonal Antibody (bsm-52131R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1(T450) (4A3) Monoclonal Antibody, Unconjugated (bsm-52131R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1(T450) (4A3) Monoclonal Antibody, Unconjugated (bsm-52131R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1(T450) (4A3) Monoclonal Antibody, Unconjugated (bsm-52131R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52131R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Embryo lysates; Lane 2: Mouse Lung lysates; Lane 3: A549 lysates; Lane 4: Mouse Cerebrum lysates probed with AKT1(Thr34) Polyclonal Antibody, Unconjugated (bs-5194R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with phospho-AKT1(Thr34) Polyclonal Antibody, Unconjugated (bs-5194R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human Siha cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Adrenal gland lysates; Lane 4: Mouse Skeletal muscle lysates; Lane 5: Mouse Ovary lysates; Lane 6: Mouse Lung lysates; Lane 7: Mouse Cerebrum lysates; Lane 8: Mouse Placenta lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human A549 cell lysates; Lane 11: Mouse Heart cell lysates probed with AKT1 Polyclonal Antibody, Unconjugated (bs-5194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5194R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti phospho-AKT1\/2\/3 (Tyr315\/316\/312) Polyclonal Antibody, Unconjugated (bs-5193R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.
Lane 1: Mouse Adrenal glands lysates; Lane 2: Mouse Cerebrum lysates probed with phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Polyclonal Antibody, Unconjugated (bs-5193R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) ) polyclonal Antibody, Unconjugated (bs-5193R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5193R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HepG2 lysate probed with Rabbit Anti-AKT1+2+3 Polyclonal Antibody (bs-6951R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse heart lysates probed with AKT1+2+3 Polyclonal Antibody, Unconjugated (bs-6951R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Lung lysates probed with AKT1+2+3 Polyclonal Antibody, Unconjugated (bs-6951R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT1+2+3) Polyclonal Antibody, Unconjugated (bs-6951R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6951R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Flow cytometric analysis of A549 cells with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
MCF-7 cell lysate probed with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52468R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.
U937 cell lysates probed with Anti-AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0876R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Lane 1: Human HUVEC cell lysates; Lane 2: Human A431 cell lysates probed with AKT1\/2\/3 Polyclonal Antibody, Unconjugated (bs-5209R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5209R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Akt1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.

Catalog #1: bs-5182R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human myometrium labeled with Anti-phospho-AKT1 (Tyr474)Polyclonal Antibody, Unconjugated (bs-10133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
Mouse lung lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n

Catalog #1: bs-10133R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated