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Lane 1: Hela Cells; Lane 2: Mouse spleen; Probed with Cystatin C (5C2) Monoclonal Antibody (bsm-52759R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Cystatin C (5C2) Monoclonal Antibody (bsm-52759R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Cystatin C (5C2) Monoclonal Antibody (bsm-52759R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Cystatin C (5C2) Monoclonal Antibody (bsm-52759R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52759R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10708R

Applications: IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

A431 cell lysates probed with Cytokeratin 5 (7B12) Monoclonal Antibody, Unconjugated (bsm-52340R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CytokeRatin 5 (7B12) Monoclonal Antibody (bsm-52340R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with CytokeRatin 5 (7B12) Monoclonal Antibody (bsm-52340R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with CytokeRatin 5 (7B12) Monoclonal Antibody (bsm-52340R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52340R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 8 Monoclonal Antibody, Unconjugated (bsm-54512R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 8 Monoclonal Antibody, Unconjugated (bsm-54512R) at 1:50 for 30 minutes at room temperature, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-54512R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (\r\nCytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-54512R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54512R

Applications: WB, FCM, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Hela cell; Lane 2: MCF-7 cell; Lane 3: A431 cell; Probed with Cytokeratin 8 + 18 (8C12) Monoclonal Antibody (bsm-52419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with Cytokeratin 8 + 18 (8C12) Monoclonal Antibody (bsm-52419R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with CytokeRatin 8+18 (8C12) Monoclonal Antibody (bsm-52419R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CytokeRatin 8+18 (8C12) Monoclonal Antibody (bsm-52419R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52419R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-54606R

Applications: WB, IHC-P, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human 293T cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates probed with Cytokeratin-8 Polyclonal Antibody, Unconjugated (bs-55051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Cytokeratin-8 knockout (KO) HeLa cell lysates probed with Cytokeratin-8 Polyclonal Antibody, Unconjugated (bs-55051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cytokeratin-8) polyclonal Antibody, Unconjugated (bs-55051R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin-8 Polyclonal Antibody, Unconjugated (bs-55051R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55051R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-70058R

Applications: WB, IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysate probed with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DARPP32 (6A12) Monoclonal Antibody (bsm-52416R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52416R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-42287r-100ul

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-55053R

Applications: WB, IP, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1:Human skeletal muscle lysates; Lane 2: Mouse heart lysates probed with Desmin (5A5) Monoclonal Antibody, Unconjugated (bsm-52363R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse bladder tissue incubated with Desmin (5A5) Monoclonal Antibody (bsm-52363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52363R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 21kD. Observed band size: 21kD.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DHFR Polyclonal Antibody, Unconjugated (bs-9058R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bs-9058r-100ul

Applications: IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: MCF-7 Cells; Probed with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:100 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52544R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
MCF-7 cell lysate probed with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52532R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-25502r-100ul

Applications: IHC

Reactivity: Mouse

Conjugate: Unconjugated

Human liver lysates probed with DNAJC15 (8F11) Monoclonal Antibody (bsm-52395R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with DNAJC15 (8F11) Monoclonal Antibody (bsm-52395R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with DNAJC15 (8F11) Monoclonal Antibody (bsm-52395R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with DNAJC15 (8F11) Monoclonal Antibody (bsm-52395R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52395R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70069R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Non-Human Primate

Conjugate: Unconjugated

Catalog #1: bs-70070R

Applications: WB, IHC

Reactivity: Human, Non-Human Primate

Conjugate: Unconjugated

Catalog #1: bsm-70072M

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Mouse kidney cells lysates probed with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with DRD1 (3C3) Monoclonal Antibody (bsm-52920R) at 1:200 in 293T cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52920R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70073R

Applications: WB, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse spleen Probed with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52770R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF-7 cell lysate probed with DYNLL1 (9T1) Monoclonal Antibody (bsm-52688R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat esophagus tissue incubated with DYNLL1 (9T1) Monoclonal Antibody (bsm-52688R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52688R

Applications: WB, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human skeletal muscle lysates probed with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Dysferlin (7C1) Monoclonal Antibody (bsm-52825R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52825R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-70360M

Applications: WB, IP, IHC, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
HepG2 cell lysate probed with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with E2F1 (3C2) Monoclonal Antibody (bsm-52760R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52760R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF-7 cells Probed with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with EBP50 (3C3) Monoclonal Antibody (bsm-52784R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52784R

Applications: WB, IHC-P, IHC

Reactivity: Human, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with EDG1 (1C1) Monoclonal Antibody (bsm-52902R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
SH-SY5Y cells lysates probed with EDG1 (1C1) Monoclonal Antibody (bsm-52902R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52902R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55058R

Applications: WB, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: HL-60 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse pancreas lysates probed with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55059R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: 293T; Lane 3: Human skin lysate probed with EGFR (3F5) Monoclonal Antibody, unconjugated (bsm-52334R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
Flow cytometric analysis of A431 cells with EGFR (3F5) Monoclonal Antibody (bsm-52334R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with EGFR (3F5) Monoclonal Antibody (bsm-52334R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with EGFR (3F5) Monoclonal Antibody (bsm-52334R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52334R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EGLN1) polyclonal Antibody, Unconjugated (bs-55063R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55063R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates probed with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Eif2ak2 ) polyclonal Antibody, Unconjugated (bs-55064R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55064R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of nih\/3t3 cells with eIF4EBP1 (9C1) Monoclonal Antibody (bsm-52771R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: 293 Cells; Probed with eIF4EBP1 (9C1) Monoclonal Antibody (bsm-52771R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with eIF4EBP1 (9C1) Monoclonal Antibody (bsm-52771R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with eIF4EBP1 (9C1) Monoclonal Antibody (bsm-52771R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52771R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Mouse fat lysates; Lane 6: Mouse heart lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: EIF4EBP1 knockout (KO) HeLa cell lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EIF4EBP1) polyclonal Antibody, Unconjugated (bs-55065R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55065R

Applications: WB, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Jurkat Cells; Lane 2: MCF-7 Cells; Probed with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52519R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55067R

Applications: WB, IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: HCT116 cells; Lane 2: SW480 cells; Probed with EpCAM (6H5) Monoclonal Antibody (bsm-52417R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with EpCAM (6H5) Monoclonal Antibody (bsm-52417R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with EpCAM (6H5) Monoclonal Antibody (bsm-52417R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon tissue incubated with EpCAM (6H5) Monoclonal Antibody (bsm-52417R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52417R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60797r-100ul

Applications: IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-72141M

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72142M

Applications: WB, ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72146M

Applications: WB, IHC

Reactivity:

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 \u03bcg\/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ER\u03b1 (left) or ER\u00df1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 \u03bcg\/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 \u03bcg of antibody were used per ChIP experiment. Recovery (%: ChIP\/input) and occupancy (x fold: +ve\/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #1: bsm-53007M

Applications: WB, IHC-P, IHC, dot-blot

Reactivity: Human, Monkey

Conjugate: Unconjugated

Catalog #1: bs-51007R

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Jurkat cell lysates probed with ERG (3F9) Monoclonal Antibody, unconjugated (bsm-52324R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52324R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70084R

Applications: WB, IHC, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: Jurkat; Lane 3: K562 lysate probed with \r\nERK1 (1B9) Monoclonal Antibody (bsm-52322R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with ERK1 (1B9) Monoclonal Antibody (bsm-52322R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ERK1 (1B9) Monoclonal Antibody (bsm-52322R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with ERK1 (1B9) Monoclonal Antibody (bsm-52322R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52322R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated