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Catalog #1: bs-6983R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy7

Catalog #1: bs-6983R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Lane 1: SiHa Cells; Probed with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54266R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of SH-SY5Y cells with Wnt5a (6C2) Monoclonal Antibody (bsm-54183R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Wnt5a (6C2) Monoclonal Antibody (bsm-54183R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with Wnt5a (6C2) Monoclonal Antibody (bsm-54183R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Lane 1: Hela Cells; Probed with Wnt5a (6C2) Monoclonal Antibody (bsm-54183R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-54183R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:1000 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-WNT5A Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1948R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-1948R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 350

Catalog #1: bs-1948R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 405

Catalog #1: bs-1948R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 488

Catalog #1: bs-1948R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 555

Catalog #1: bs-1948R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 594

Catalog #1: bs-1948R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 647

Catalog #1: bs-1948R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 680

Catalog #1: bs-1948R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 750

Catalog #1: bs-1948R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC

Catalog #1: bs-1948R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5

Catalog #1: bs-1948R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5.5

Catalog #1: bs-1948R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy7

Catalog #1: bs-1948R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-1948R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-1948R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-1948R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-1948R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-1948R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-1948R-PE-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy3

Catalog #1: bs-1948R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5

Catalog #1: bs-1948R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5.5

Catalog #1: bs-1948R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy7

Catalog #1: bs-1948R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP

Catalog #1: bs-1948R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy5.5

Catalog #1: bs-1948R-PerCP-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy7

Catalog #1: bs-1948R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: RBITC

Catalog #1: bsm-54708R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: MCF-7, 2: Mouse ovary, 3: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 47 kDa\r\nObserved MW: 47 kDa
Tissue: Human testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60630R
Tissue: Human cerebrum\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60630R
Tissue: rat cerebrum\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60630R

Catalog #1: bsm-60630R

Applications: WB, FCM, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-WWOX Polyclonal Antibody (bs-0589R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse cerebrum lysates probed with WWOX Polyclonal Antibody, Unconjugated (bs-0589R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WWOX Polyclonal Antibody, Unconjugated (bs-0589R) at 1:200 overnight at 4\u00b0C, DAB staining.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nWWOX Antibody(bs-0589R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0589R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of 293T cells with XBP1 (2C5) Monoclonal Antibody (bsm-52898R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2 cells lysates; Lane 2: 293T brain cells lysates; Probed with XBP1 (2C5) Monoclonal Antibody (bsm-52898R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52898R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-XBP-1 Polyclonal Antibody, Unconjugated (bs-1668R) followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysate L2 mouse liver lysates probed with Anti XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 29kD. Observed band size: 29kD
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Testis lysates; Lane 4: HepG2 cell lysates probed with XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (bs-1668R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1668R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1668R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 350

Catalog #1: bs-1668R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-1668R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-1668R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-1668R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-1668R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-1668R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-1668R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-1668R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-1668R-APC-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5

Catalog #1: bs-1668R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bs-1668R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7