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Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1\/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
HeLa cells were stained with AQP1 Polyclonal Antibody, Unconjugated(bs-1506R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Human 293T cells probed with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).\\n

Catalog #1: bs-1506R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1506R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1506R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody (bs-4611R) at 1:50 for 40 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) FITC Conjugated Secondary Antibody.
Image provided the Independent Validation Program, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated (bs-4611R) at 1:200 overnight at 37\u00b0C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) at 1:200, 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue,C-0033) was used to stain the cell nuclei.\\n

Catalog #1: bs-4611R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T Cells; Probed with AQP2 Polyclonal Antibody, unconjugated (bs-0261R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse kidney lysates; Lane 2: 293T cell lysates probed with AQP2 Polyclonal Antibody, Unconjugated (bs-0261R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0261r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4611R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0261r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4611R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0261r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-5185R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4610R

Applications: WB, ELISA

Reactivity: Dog

Conjugate: Unconjugated

Catalog #1: bs-4610R-Biotin

Applications: WB, ELISA

Reactivity: Dog

Conjugate: Biotin

Catalog #1: bs-4610R-HRP

Applications: WB, ELISA

Reactivity: Dog

Conjugate: HRP

Catalog #1: bs-5187R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-5187R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-5187R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-AQP-3 Polyclonal Antibody, Unconjugated(bs-1253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rat tongue tissue labeled with Anti-AQP-3 Polyclonal Antibody, Unconjugated(bs-1253R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with AQP3 Polyclonal Antibody, Unconjugated (bs-1253R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1253R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1253R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1253R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
A549 cells probed with AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-1554R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1554R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1554R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
A549 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nAQP7 Antibody\uff08bs-2506R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-2506R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-2506R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bs-2506R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Mouse liver lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse intestine lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2060R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-2060R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Others

Conjugate: Biotin

Catalog #1: bs-2060R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Others

Conjugate: HRP

Catalog #1: bs-10927R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10927R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10927R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3751R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3751R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-3751R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-5 lipoxygenase\/ALOX5 Polyclonal Antibody, Unconjugated (bs-0526R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Arachidonate 5-lipoxygenase Polyclonal Antibody, Unconjugated (bs-0526R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ALOX5\/5 lipoxygenase Antibody(bs-0526R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0526R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0526R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0526R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-ARC Polyclonal Antibody (bs-0385R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
A549 cells probed with Rabbit Anti-ARC\/Arg3.1 Polyclonal Antibody (bs-0385R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Lane 1: Rat brain; Lane 2: mouse brain lysate probed with ARC Polyclonal Antibody, Unconjugated (bs-0385R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0385R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0385R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0385R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-12508R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-12508R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-12508R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-12509R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1145R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1145R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-1145R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-3006R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated