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IF (IHC-P) Antibodies

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Arctic Ground Squirrel splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Rat splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Lane 1: Mouse heart lysates Lane 2: Mouse liver lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-3614R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: Unconjugated

Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1\/Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-1353R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat intestine labeled with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Free Sample Program. Mouse pancreatic cancer tissue lysates probed with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated (bs-10005R) at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody and DAB development.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody (bs-10005R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody (bs-10005R) at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-10005R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0107R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD200\/MOX1 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD200\/MOX1 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human Colon Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.

Catalog #1: bs-6030R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with IL-6R Beta\/CD130\/gp130 Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human Raji cells probed with IL-6R beta Polyclonal Antibody, Unconjugated (bs-1459R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-1459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-NGF Polyclonal Antibody, Unconjugated (bs-0067R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (bs-0067R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0067R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat liver tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody(bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Substance P Polyclonal Antibody, Unconjugated (bs-0065R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0065R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-BFGF\/FGF2 Polyclonal Antibody, Unconjugated (bs-0217R) followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
HepG2 lysates probed with bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0217R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IL-10 Polyclonal Antibody, Unconjugated (bs-6761R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL-10 Polyclonal Antibody, Unconjugated (bs-6761R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-6761R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human K562 lysates, Lane 2: Human A431 lysates probed with Rabbit Anti-GDF15\/MIC-1 Polyclonal Antibody, Unconjugated (bs-3818R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Urinary bladder lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Rat Urinary bladder lysates; Lane 4: Rat Large intestine lysates; Lane 5: Human HT1080 cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human K562 cell lysates; Lane 8: Human A549 cell lysates probed with GDF15\/MIC-1 Polyclonal Antibody, Unconjugated (bs-3818R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3818R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:1000 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-WNT5A Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1948R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4\/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4\/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse macrophages(RAW264.7)labeled with Rabbit Anti TLR4\/CD284 Polyclonal Antibody, Unconjugated (bs-1021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image submitted by One World Lab validation program. HL60 and MCF-7 cells were stained with rabbit polyclonal antibody against TLR4 with two dilutions (1:100 and 1:250). 2nd antibody without primary antibody was used as control included here.

Catalog #1: bs-1021R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat retina labeled with Anti-MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell lysates probed with MTNR1B Polyclonal Antibody, Unconjugated (bs-0963R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (bs-0963R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0963R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Formalin-fixed and rat pancreas tissue labeled with Anti-FN\/Fibronectin 1 Polyclonal Antibody, Unconjugated (bs-0666R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)
Image kindly submitted by Edita Aksamitiene as part of the free sample program. Rat skin lysates probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:1000.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Fibronectin Polyclonal Antibody (bs-0666R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded rat intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated (bs-0666R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-0666R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Unconjugated

Catalog #1: bs-0813R

Applications: WB, ELISA, FCM, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Bovine, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
U-87MG cells were fixed with 4% PFA for 10min at room temperature \uff0cpermeabilized with 20% PBST for 20 min at room temperature , and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PERK Polyclonal Antibody at 1\uff1a50 dilution in blocking buffer and incubated for 30 min at -20\u2103, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2469R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U251 cells were stained with ATF6 Polyclonal Antibody (bs-1634R) at 1:200 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Pancreas lysates probed with ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Huvec cell lysates probed with ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1634R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

L1 rat brain lysates L2 rat heart lysates probed with Anti ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 46kD. Observed band size: 46kD
Mouse splenoctyes probed with Rabbit Anti-ADRB2 Polyclonal Antibody (bs-0947R) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Stomach tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Human A431 cell lysates probed with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0947R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded rat lung labeled with Rabbit Anti-AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
A549 cells probed with AQP5 Polyclonal Antibody, Unconjugated (bs-1554R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-1554R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1559R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-BMP2 Polyclonal Antibody, Unconjugated (bs-1012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti BMP2 Polyclonal Antibody, Unconjugated (bs-1012R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 44kD. Observed band size:44kD.\\n
Lane 1: Human U-2OS cell lysates; Lane 2: Human MG63 cell lysates; Lane 3: Human HUVEC cell lysates probed with BMP2 Polyclonal Antibody, Unconjugated (bs-1012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0886R

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) used at 1:200 dilution for 40 minutes at 37\u00b0C

Catalog #1: bs-2934R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: EC-9706 cell lysates; Lane 2: MCF-7 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10316R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human hepatoma labeled with Anti-TRT\/TERT Polyclonal Antibody, Unconjugated (bs-0233R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:200 overnight at 4\u00b0C, DAB staining.
A375 lysates probed with TERT Polyclonal Antibody, Unconjugated (bs-0233R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0233R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-phospho-HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: MCF-7 Cells; Probed with HMGCR(Ser872) Polyclonal Antibody, Unconjugated (bs-4063R) at 1:1000, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.
An ELISA was performed using a serial dilution of HMGCR (Ser872) Polyclonal Antibody (bs-4063R) in antigen-coated wells. The antigen used was a peptide containing the phosphosite of interest. The plot shows the absorbance against the antibody dilution for phosphopeptide antigen and non-phosphorylated peptide antigen.

Catalog #1: bs-4063R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

293T lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Rat Spleen tissue lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human THP-1 cell lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2955R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

RSC96 cells probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Bone lysates probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD47) polyclonal Antibody, Unconjugated (bs-2386R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2386R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse brain lysates L2 mouse heart lysates probed with Anti MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 78kD. Observed band size: 78kD.

Catalog #1: bs-4593R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti BRN3A Polyclonal Antibody, Unconjugated (bs-3669R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3669R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144\/VECD\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4\u00b0C, The secondary antibody was diluted to 1:100 for 120 minutes at 37\u00b0C.
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0878R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0163R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-Osteoprotegerin Polyclonal Antibody (bs-0431R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse heart lysates; Lane 2: Mouse kidney lysates; Lane 3: Mouse liver lysates probed with OPG Polyclonal Antibody (bs-0431R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Lane 1: Human Raji cell lysates; Lane 2: Human U251 cell lysates probed with OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0431R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated(bs-0648R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.
Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody (bs-0648R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse lymph node lysates probed with CD8 Polyclonal Antibody, unconjugated (bs-0648R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0648R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-6763R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti-PRSS10 Polyclonal Antibody, Unconjugated (bs-6285R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Du145 cell lysates; Lane 2: Mouse Placenta lysates probed with PRSS10\/TMPRSS2 Polyclonal Antibody, Unconjugated (bs-6285R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6285R

Applications: WB, ELISA, IHC-P, IHC-F, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
RSC96 probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1970R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Mouse lung and lymph node lysates probed with Rabbit Anti-CRLR\/CGRPR1 Polyclonal Antibody, Unconjugated (bs-1860R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Heart tissue lysates probed with CALCRL Polyclonal Antibody, Unconjugated (bs-1860R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1860R

Applications: WB, ELISA, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4563R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebral cortex; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuN Polyclonal Antibody, Unconjugated bs-1613R at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1613R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded cervical cancer labeled with Anti-Integrin \u03b1V+\u03b25 Polyclonal Antibody, Unconjugated (bs-1356R) followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with Rabbit Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody (bs-1356R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bs-1356R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal cancer labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysate probed with Anti-CTGF Polyclonal Antibody (bs-0743R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

Catalog #1: bs-0743R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RNF43 Polyclonal Antibody, Unconjugated(bs-7007R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse testis labeled with Anti RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Kidney lysates; Lane 3: Mouse Heart lysates; Lane 4: Rat Cerebrum lysates ; Lane 5: Rat Kidney lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7007R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat aorta labeled with Anti-LOX-1 Polyclonal Antibody, Unconjugated (bs-2044R) followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with Rabbit Anti-LOX1 Polyclonal Antibody, Unconjugated (bs-2044R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
This image was generously provided by Zu Yue Deng from Zhejiang University of Technology. Human heart tissue probed with Rabbit Anti-LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R). Protein bands were developed using an HRP system.
RSC96 cells probed with LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-2044R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-1665R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated