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Tissue\/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded; \r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37\u2103 for 20 min; \r\nIncubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1278R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6313R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163\/M130 Polyclonal Antibody, Unconjugated( bs-2527R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163\/M130 Polyclonal Antibody, Unconjugated (bs-2527R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163\/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37\u00b0C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37\u00b0C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \\n

Catalog #1: bs-2527R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Unconjugated

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat aorta, brain, spleen, pancreas, muscle, bladder, lungs, liver, and kidney lysates probed with Rabbit Anti-Collagen I\uffa0Polyclonal antibody (bs-10423R)at 1:1000.
Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen I\uffa0Polyclonal antibody (bs-10423R)at 1:1000.
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-10423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was kindly submitted by Kandy Velazquez of USC School of Medicine.\r\nMouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.

Catalog #1: bs-10423R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Rat lung lysate 30ug, probed (bs-0061R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 23kD. Observed band size:23kD.

Catalog #1: bs-0782R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (bs-0737R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 92kD
KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-0737R) followed by a FITC Conjugated Secondary Antibody.
Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat brain and spleen lysates probed with Rabbit Anti-HIF-1 Alpha Polyclonal Antibody (bs-0737R) at 1:1000.

Catalog #1: bs-0737R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #1: bs-0081R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon tissue labeled Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse spleen lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody\r\nincubation at 1:20000 for 60 min at 37˚C.\r\n
Lane 1: Human THP-1 cell lysates; Lane 2: Human THP-1-LPS\r\ncell lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0698R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium labeled with Rabbit Anti-Oxytocin R Polyclonal Antibody, Unconjugated (bs-1314R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Ovary lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebrum lysates probed with Oxytocin R Polyclonal Antibody, Unconjugated (bs-1314R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1314R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat renal tissue labeled with Anti-TGF-beta-1 Polyclonal Antibody, Unconjugated(bs-0086R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Isolated Transforming growth factor beta-1; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Embryo; Lane 2: Rat Cerebrum; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4˚C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0086R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated (bs-0199R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human placenta tissue labeled with Anti-GFAP Polyclonal Antibody, Unconjugated (bs-0199R) at 1:800, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-GFAP Polyclonal Antibody (bs-0199R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-GFAP Polyclonal Antibody (bs-0199R) at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-0199R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cells labeled with Anti-VEGF Polyclonal Antibody (bs-0279R).
Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 23,46kDa
HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\\n

Catalog #1: bs-0279R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Pig, Goat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse myocardium labeled with (bs-1074R) Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 66kD. Observed band size:66kD.\\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1074R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody

Catalog #1: bs-2013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1\/CD90\/ Thy1.1 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0778R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney cells labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded Lymphocytes labeled with (bs-0766R) Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated followed by incubation with a conjugated secondary antibody.
Formalin-fixed and paraffin embedded Lymphocytes labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) followed by incubation with a conjugated secondary antibody and DAPI staining.

Catalog #1: bs-0766R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-MTNR1A\/MTR-1A\/MEL-1A-R Polyclonal Antibody, Unconjugated (bs-0027R) at 1:300 followed by conjugation to the secondary antibody
Lane 1: Cerebrum lysates probed with Melatonin Receptor 1A Antibody (bs-0027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Melatonin Receptor 1A Polyclonal Antibody(bs-0027R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.

Catalog #1: bs-0027R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Unconjugated

Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 44kD
Formalin-fixed and paraffin embedded human liver labeled with Anti-phospho-ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1\/2(Thr202 + Tyr204)Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3016R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Unconjugated

Arctic Ground Squirrel splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Rat splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Lane 1: Mouse heart lysates Lane 2: Mouse liver lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-3614R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody (bs-0065R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat liver tissue labeled with Rabbit Anti-Substance P Polyclonal Antibody(bs-0065R)at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Substance P Polyclonal Antibody, Unconjugated (bs-0065R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0065R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-0813R

Applications: WB, ELISA, FCM, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Bovine, Others

Conjugate: Unconjugated

Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-0163R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Rabbit Anti-CD118 Polyclonal Antibody (bs-1458R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
MCF-7 lysates probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1458R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-LAMP2\/CD107B Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2379R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Others

Conjugate: Unconjugated

Catalog #1: bs-1126R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne. Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:300 followed by conjugation to a secondary antibody and staining.
Lane 1: Mouse Testis lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Testis lysates; Lane 4: Rat Cerebrum lysates probed with GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0654R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Unconjugated

Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti Smad3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Ovary lysates; Lane 3: HT1080 cell lysates; Lane 4: Jurkat cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2\/3 Polyclonal Antibody(bs-3484R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Skin lysates; Lane 5: Mouse Kidney lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Kidney lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human A549 cell lysates; Lane 11: Human Hela cell lysates; Lane 12: Human HT1080 cell lysates; Lane 13: Human A431 cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3484R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig, Others

Conjugate: Unconjugated

RSC96 cells probed with Rabbit Anti-DAT\/Dopamine transporter Polyclonal Antibody (bs-1714R ) at 6ug for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dopamine Transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat Cerebrum lysates probed with Dopamine Transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DAT\/Dopamine transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1714R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human A431 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Rat Ear lysates; Lane 4: Mouse Ear lysates; Lane 5: Mouse Urinary bladder lysates probed with Integrin beta 1 Polyclonal Antibody, Unconjugated (bs-0486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Integrin beta 1) polyclonal Antibody, Unconjugated (bs-0486R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0486R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Unconjugated

Antigen: bs-10446P, 0.2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; TMB staining; Read the data in MicroplateReader by 450nm.
Hela (Positive) and RSC96 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha HPV16-E7Antibody (bs-10446R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
Lane 1: Recombinant HPV16 E7 protein Bacterial lysate, DsbC & His(bs-49101L) probed with HPV16 E7 Polyclonal Antibody, Unconjugated (bs-10446R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10446R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human heart tissue labeled with Anti-PAX7 Polyclonal Antibody, Unconjugated (bs-2413R) followed by conjugation to the secondary antibody
Human HepG2 cells probed with Polyclonal Antibody, unconjugated (bs-2413R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n

Catalog #1: bs-2413R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Sheep, Others

Conjugate: Unconjugated

Catalog #1: bs-6988R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Plant, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated(bs-0349R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0349R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Antigen: bs-2033P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: E.coli lysates; Lane 2: E.coli lysates; Probed with E.coli DH-5 Alpha Polyclonal Antibody, Unconjugated (bs-2033R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2033R

Applications: WB, ELISA, IF(IHC-P)

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Tyrosine Hydroxylase(Neuronal Marker)\/Tyk2\/TYH Polyclonal Antibody, Unconjugated (bs-0016R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
RSC96 probed with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 6ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human PC-3 lysates probed with Tyrosine Hydroxylase Polyclonal Antibody, unconjugated (bs-0016R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (bs-0016R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0016R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10\u03bcM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.

Catalog #1: bs-0489R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Others

Conjugate: Unconjugated

Lane 1: mouse brain lysates probed with Rabbit Anti-PRLR Polyclonal Antibody, Unconjugated (bs-6445R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-PRLR Polyclonal Antibody, Unconjugated (bs-6445R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with PRLR Polyclonal Antibody (bs-6445R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-6445R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled Anti-HPV16 E6 + HPV18 E62 Polyclonal Antibody, Unconjugated (bs-1719R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-1719P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.1ug Lysates; Lane 2: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.05ug Lysates. Probed with HPV16 E6 + HPV18 E6 polyclonal Antibody, unconjugated (bs-1719R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with HPV16 E6 + HPV18 E6 Polyclonal Antibody(bs-1719R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1719R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-10180R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Virus, Others

Conjugate: Unconjugated

Lane 1: Huh7 probed with Rabbit Anti-CD44 Polyclonal Antibody, Unconjugated (bs-0521R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-CD44 Polyclonal Antibody (bs-0521R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human Raji cells probed with CD44 Polyclonal Antibody, Unconjugated (bs-0521R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human PANC-1 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human A549 cell lysates probed with CD44 Polyclonal Antibody, Unconjugated (bs-0521R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0521R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP19\/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CYP19\/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with Aromatase Polyclonal Antibody, Unconjugated (bs-1292R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1292R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- Bax Polyclonal Antibody, Unconjugated (bs-0127M) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
L1 mouse cerebrum lysates, probed: Anti-Bax (bs-0127M) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:2000 90min in 37˚C. Predicted and observed band size: 21kD
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0127M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat carotid artery Anti-MMP-2 Polyclonal Antibody (bs-0412R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mmp2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0412R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Unconjugated