phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Recombinant Antibody

The Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63322R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

The Jurkat (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63322R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

The C6 (treated with 200 ng\/ml PMA for 30 minutes) (Rat) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187) antibody (bsm-63322R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

4% Paraformaldehyde-fixed HeLa (treated with 200 ng\/ml TPA\/PMA for 30 minutes) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-ERK1 (T202\/Y204)\/ERK2 (T185\/Y187)) monoclonal Antibody, unconjugated (bsm-63322R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.