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OPG Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OPG Monoclonal Antibody, Unconjugated(bsm-62482R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

Applications

  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-62482r
Product Name OPG Recombinant Antibody
Applications IHC-P, IHC-F, IF(ICC), IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TNFRSF11B
Clonality Monoclonal
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4982
Swiss Prot O00300
Synonyms OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; TNFRSF11B; TR1.
Background Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B.
Application Dilution
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

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