CHRM3 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CHRM3 monoclonal antibody, unconjugated (bsm-61645R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CHRM3 monoclonal antibody, unconjugated (bsm-61645R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-61645r
Product Name	CHRM3 Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHRM3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1131
Swiss Prot	P20309
Subcellular location	Cell membrane
Synonyms	AChR; CHRM3; EGBRS; HM3; M3 muscarinic receptor.
Background	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Application Dilution
WB	1:300-5000