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NLRP3 Recombinant Antibody

Western blot analysis of SH-SY5Y cell lysate. Using NLRP3 (bsm-61254R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Western blot analysis of SH-SY5Y cell lysate. Using NLRP3 (bsm-61254R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61254r
Product Name NLRP3 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF(ICC), IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NLRP3
Clonality Monoclonal
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 114548
Swiss Prot Q96P20
Subcellular location Cytoplasm
Synonyms FCU; MWS; FCAS; Cias1; Mmig1; NLRP3; Pypaf1; AII/AVP; AGTAVPRL; Cryopyrin.
Background Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

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