NLRP3 Recombinant Antibody

$4% Paraformaldehyde-fixed THP-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NLRP3) monoclonal Antibody, unconjugated (bsm-61254R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed THP-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NLRP3) monoclonal Antibody, unconjugated (bsm-61254R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61254r
Product Name	NLRP3 Recombinant Antibody
Applications	ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NLRP3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	114548
Swiss Prot	Q96P20
Subcellular location	Cytoplasm
Synonyms	FCU; MWS; FCAS; Cias1; Mmig1; NLRP3; Pypaf1; AII/AVP; AGTAVPRL; Cryopyrin.
Background	Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis.
Application Dilution
ICC/IF	1:50-100