CD8 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61078r-bf647-100uL |
| Product Name | CD8 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD8 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 925 |
| Synonyms | CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen. |
| Background | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Application Dilution | |
| IF(IHC-P) | 1:200-1000 |
Frequently asked questions
Which applications is CD8 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61078R-BF647) validated for, and at what dilution?
bsm-61078R-BF647 is validated for IF(IHC-P)(IF(IHC-P)) (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD8 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61078R-BF647) react with?
Tested reactivity: Human.
How should bsm-61078R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD8 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61078R-BF647)?
KLH conjugated synthetic peptide derived from human CD8
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61078R-BF647)?
AbBy Fluor-647 on bsm-61078R-BF647: excitation / emission 650nm/665nm.