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COX4I1 Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse \r\nheart\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 20 kDa\r\nObserved MW: 15 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: MEF, 3: C6, 4: Mouse heart Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 20 kDa Observed MW: 15 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60712r
Product Name COX4I1 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human COX IV
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1327
Swiss Prot P13073
Subcellular location Cytoplasm, Cell membrane
Synonyms COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

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