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phospho-IKB Alpha (Ser32 / Ser36) Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+ Human \u03b1-TNF \r\n(20ng\/ml, 30min)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 36 kDa\r\nObserved MW: 36 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+ Human α-TNF (20ng/ml, 30min) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 36 kDa Observed MW: 36 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60711r
Product Name phospho-IKB Alpha (Ser32 / Ser36) Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36)
Modification Site Ser32 / Ser36
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4792
Swiss Prot Q9Z1E3
Subcellular location Cytoplasm, Nucleus
Synonyms NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN; I_B-_; I_B_.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:50-100
IF IF=1:1:50-100

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