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ASGPR Monoclonal Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HepG2, 2: Human liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 33 kDa\r\nObserved MW: 40-50 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: Human liver Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 33 kDa Observed MW: 40-50 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-60428r-100ul
Product Name ASGPR Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Monoclonal
Clone # H12B5
Isotype IgG
Concentration 1mg/ml
Purification Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P07306
Application Dilution
WB =1:200-1:1000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=0
Flow-Cyt Flow-Cyt=1:20-1:100
ICC/IF ICC/IF=1:20-1:50

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