alpha Actinin 4 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-60423r-100uL |
| Product Name | alpha Actinin 4 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human alpha Actinin 4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 81 |
| Swiss Prot | O43707 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | actinin 4; Actinin alpha 4; Actinin alpha-4; Alpha-actinin-4; _-Actinin-4; _Actinin-4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4. |
| Background | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:50-1:100 |
| IHC-F | ICC=1:50 |
| IF | IF=1:100-500 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is alpha Actinin 4 Recombinant Antibody (bsm-60423R) validated for, and at what dilution?
bsm-60423R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:50-1:100); IHC-F (ICC=1:50); IF (IF=1:100-500); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha Actinin 4 Recombinant Antibody (bsm-60423R) react with?
Tested reactivity: Human, Mouse.
How should bsm-60423R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise alpha Actinin 4 Recombinant Antibody (bsm-60423R)?
KLH conjugated synthetic peptide derived from human alpha Actinin 4