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alpha Actinin 4 Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1:HeLa, 2:HEK-293, 3:4T1, 4:Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 100 kDa\r\nObserved MW: 110 kDa\r\n
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1:HeLa, 2:HEK-293, 3:4T1, 4:Raw264.7 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 100 kDa Observed MW: 110 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-60423r
Product Name alpha Actinin 4 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human alpha Actinin 4
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 81
Swiss Prot O43707
Subcellular location Cytoplasm, Nucleus
Synonyms actinin 4; Actinin alpha 4; Actinin alpha-4; Alpha-actinin-4; _-Actinin-4; _Actinin-4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4.
Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].
Application Dilution
WB =1:200-1000
IHC-P IHC-P=1:20-1:100
IHC-F IHC-F=1:400-800
IF IF=1:20-1:100
ICC/IF ICC/IF=1:20-1:100

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