HDAC3 Monoclonal Antibody

Name: HDAC3 Monoclonal Antibody
Brand: Bioss
SKU: bsm-60265M
Price: 490.0 USD
Availability: InStock

$The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60265M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60265M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC3) monoclonal Antibody, unconjugated (bsm-60625M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC3) monoclonal Antibody, unconjugated (bsm-60625M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

IF(ICC)
FCM

Reactivity

Human

Overview
Catalog #	bsm-60265m-100uL
Product Name	HDAC3 Monoclonal Antibody
Applications	IF(ICC) FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	C12C4
Isotype	IgG2A/kappa
Concentration	Lot Dependent
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	O15379
Application Dilution
IF(ICC)	1:50-200
FCM	10ul/10^6 cells in 100ul

Frequently asked questions

Which applications is HDAC3 Monoclonal Antibody (bsm-60265M) validated for, and at what dilution?

bsm-60265M is validated for IF(ICC) (1:50-200); FCM (10ul/10^6 cells in 100ul). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HDAC3 Monoclonal Antibody (bsm-60265M) react with?

Tested reactivity: Human.

How should bsm-60265M be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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HDAC3 Monoclonal Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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