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MUC2 Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: Rat colon\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 540 kDa\r\nObserved MW: 140-170, \uff1e250 kDa\r\n
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Rat colon Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 540 kDa Observed MW: 140-170, ＞250 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bsm-60016r
Product Name MUC2 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, Flow-Cyt
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MUC2
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4583
Swiss Prot Q02817
Subcellular location Secreted
Synonyms MLP; SMUC; Intestinal mucin 2; MUC 2; MUC2; Mucin 2; Mucin 2 intestinal; Mucin 2 intestinal/tracheal; Mucin 2 oligomeric mucus/gel forming; Mucin 2 precursor; Mucin 2 tracheal; Mucin like protein; Mucin2; Intestinal mucin-2; MUC-2; Muc2; MUC2_HUMAN; Mucin-2; Mucin2.
Background This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]
Application Dilution
WB =1:500-1000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500
Flow-Cyt Flow-Cyt=1:100

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