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Insulin Recombinant Antibody

Tissue:human pancreas\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:4000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60010R
Tissue:human pancreas Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:4000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60010R

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60010r
Product Name Insulin Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Insulin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3630
Swiss Prot P01308
Subcellular location Secreted
Synonyms ILPR; INS; Insulin A chain; Insulin B chain; Insulin A chain; Insulin precursor; IRDN; Proinsulin; Proinsulin precursor; IDDM2; INS_HUMAN; MODY10.
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:1000-3000
IHC-F IHC-F=1:1000-3000
IF IF=1:1000-3000

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