Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody

Name: Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody
Brand: Bioss
SKU: bsm-54491R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Human Thyroid tumor\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Human Thyroid tumor\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Human Breast\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Human Breast\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embedded Mouse Placenta\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embedded Mouse Placenta\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$Paraformaldehyde-fixed, paraffin embeddedRat Placenta\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining$

Paraformaldehyde-fixed, paraffin embeddedRat Placenta\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated(bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining

$4% Paraformaldehyde-fixed Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-ERK1 (Thr202)+ ERK2 (Thr185)) monoclonal Antibody, unconjugated (bsm-54491R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-ERK1 (Thr202)+ ERK2 (Thr185)) monoclonal Antibody, unconjugated (bsm-54491R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The Jurkat (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Jurkat (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$The Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (treated with 200 ng\/ml PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.

$The NIH\/3T3 (treated with 200 ng\/ml PMA for 30 minutes) (M) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The NIH\/3T3 (treated with 200 ng\/ml PMA for 30 minutes) (M) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$The C6 (treated with 200 ng\/ml PMA for 30 minutes) (Rat) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The C6 (treated with 200 ng\/ml PMA for 30 minutes) (Rat) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-phospho-ERK1 (Thr202)+ ERK2 (Thr185 antibody (bsm-54491R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488):1\u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-ERK1 (Thr202)+ ERK2 (Thr185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1 (Thr202)+ ERK2 (Thr185) monoclonal antibody, unconjugated (bsm-54491R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1 (Thr202)+ ERK2 (Thr185) monoclonal antibody, unconjugated (bsm-54491R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The HeLa (treated with 200 ng\/ml TPA\/PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1\/ERK2(T202\/T185) antibody (bsm-54491R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HeLa (treated with 200 ng\/ml TPA\/PMA for 30 minutes) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-ERK1\/ERK2(T202\/T185) antibody (bsm-54491R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1\/ERK2(T202\/T185) monoclonal antibody, unconjugated (bsm-54491R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1\/ERK2(T202\/T185) monoclonal antibody, unconjugated (bsm-54491R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERK1\/ERK2 (p-T202\/T185) Monoclonal Antibody, Unconjugated (bsm-54491R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54491r-100uL
Product Name	Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody
Applications	WB FCM IHC-P IHC-F IF(IHC-P) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human ERK1 around the phosphorylation site of T202
Modification Site	Thr202+Thr185
Clonality	Recombinant
Clone #	6H2
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5594
Swiss Prot	P27361
Subcellular location	Nucleus
Synonyms	MK03_HUMAN; MAPK3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1 (ERK-1); Insulin-stimulated MAP2 kinase; MAP kinase isoform p44 (p44-MAPK); Microtubule-associated protein 2 kinase; p44-ERK1; 2.7.11.24; ERK1; PRKM3; MK01_HUMAN; MAPK1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2 \| MAPK 2); ERK2; PRKM1; PRKM2; ERK; ERK-2; MAPK2; NS13; P42MAPK; p38; p40; p41; p41mapk; p42-MAPK; ERK-1; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; p44-MAPK; mitogen-activated protein kinase 3; ERK1 & ERK2 (phospho-T202/T185); p-ERK1 & ERK2; phospho-ERK1 & ERK2; ERK1 & ERK2 (phospho-Thr202/Thr185); p44/42
Background	The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IHC-P	IHC-P1:50-200
IHC-F	IHC-F1:50-200
IF(IHC-P)	IFIHC-P1:50-200
IF(ICC)	IFICC1:50-200

Frequently asked questions

Which applications is Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody (bsm-54491R) validated for, and at what dilution?

bsm-54491R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody (bsm-54491R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54491R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody (bsm-54491R)?

A synthesized peptide derived from human ERK1 around the phosphorylation site of T202

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Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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