OPA1 (9A7) Monoclonal Antibody

Name: OPA1 (9A7) Monoclonal Antibody
Brand: Bioss
SKU: bsm-54144R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OPA1 Monoclonal Antibody, Unconjugated (bsm-54144R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with OPA1 monoclonal antibody, unconjugated (bsm-54144R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with OPA1 monoclonal antibody, unconjugated (bsm-54144R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54144r-100uL
Product Name	OPA1 (9A7) Monoclonal Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human OPA1
Clonality	Monoclonal
Clone #	9A7
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	4976
Swiss Prot	O60313
Subcellular location	Mitochondrion
Synonyms	Dynamin-like 120 kDa protein mitochondrial; Optic atrophy protein 1; OPA1; KIAA0567.
Background	OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed in the mitochondrial biogenesis. OPA1 is a cause of optic atrophy type 1. OPA1 is mostly expressed In retina but can also be expressed in brain, testis, heart and skeletal muscles. This dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission.
Application Dilution
WB	1:300-5000
IHC-F	1:50-200
IF	1:50-200
IHC-P	1:200-400

Frequently asked questions

Which applications is OPA1 (9A7) Monoclonal Antibody (bsm-54144R) validated for, and at what dilution?

bsm-54144R is validated for WB (1:300-5000); IHC-F (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OPA1 (9A7) Monoclonal Antibody (bsm-54144R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-54144R be stored?

Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OPA1 (9A7) Monoclonal Antibody (bsm-54144R)?

C terminal Human OPA1

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OPA1 (9A7) Monoclonal Antibody

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Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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