ER alpha (5G7) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-53007M-50ug |
| Product Name | ER alpha (5G7) Monoclonal Antibody |
| Applications |
WB
IHC-P
IHC
DOT-BLOT
|
| Reactivity | Human, Monkey |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Monoclonal antibody raised in mouse against the NH2 terminus of the human ER alpha (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32). |
| Clonality | Monoclonal |
| Clone # | 5G7 |
| Isotype | IgG3 |
| Concentration | 2ug/ul |
| Purification | Monoclonal antibody in PBS containing 0.05% azide; purified by ammonium sulphate precipitation followed by dialysis |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 2099 |
| Swiss Prot | P03372 |
| Synonyms | ESR, ESR1, ESRA, NR3A1 |
| Background | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC | |
| dot-blot | |
Frequently asked questions
Which applications is ER alpha (5G7) Monoclonal Antibody (bsm-53007M) validated for, and at what dilution?
bsm-53007M is validated for WB (1:300-5000); IHC-P (1:200-400); IHC; dot-blot. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ER alpha (5G7) Monoclonal Antibody (bsm-53007M) react with?
Tested reactivity: Human, Monkey.
How should bsm-53007M be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ER alpha (5G7) Monoclonal Antibody (bsm-53007M)?
Monoclonal antibody raised in mouse against the NH2 terminus of the human ER alpha (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).