NQO1 (8T5) Monoclonal Antibody

Flow cytometric analysis of Hela cells with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: SH-SY-5Y lysates; Lane 2: Mouse kidney lysates; Line 3: Raji lysates ;Probed with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY-5Y lysates; Lane 2: Mouse kidney lysates; Line 3: Raji lysates ;Probed with NQO1 (8T5) Monoclonal Antibody (bsm-52830R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52830R
Product Name	NQO1 (8T5) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	8T5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1728
Swiss Prot	P15559
Synonyms	NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1, NQO1
Background	The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Application Dilution
WB	1:300-5000
FCM	1:20-100

Applications

Reactivity

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